Yasushi OKADA (The University of Tokyo; RIKEN)
SDSRMS, the spinning disk superresolution microscope, achieved 100 frames per sec with a spatial resolution of 120 nm and 2048x2048 pixel count
|Title:||Development and application of super-resolution microscope for the high spatio-temporal live cell imaging|
|Affiliation:||Team Leader, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN; Professor , Department of Physics, Graduate School of Science, The University of Tokyo|
Dynamics of recycling endosome captured at 100 FPS, playing at 10 FPS (10x slow motion). Scale Bar, 0.5 um.
|Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. We have developed a new SR fluorescence microscope based on confocal microscope optics, which we named as the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumination microscope (SIM) and achieves a spatial resolution of 120 nm, double that of the diffraction limit of wide-field fluorescence microscopy. However, the SDSRM is 10 times faster than a conventional SIM because SR signals are recovered by optical demodulation through the stripe pattern of the disk, so that we can achieve 100 SR frames per sec with 2048x2048 pixels resolution. The principle of SDSRM and its application to live cell imaging will be presented.|
|Biographical Sketch:||Yasushi Okada is a Professor of biophysics at Department of Physics, The University of Tokyo, and a team leader in RIKEN, Quantitative Biology Center. After graduating the medical school of the University of Tokyo, he became a research associate at the Department of Cell Biology and Anatomy, The University of Tokyo and moved to RIKEN in 2011. In 2016, he was invited to the current professor position with a cross-appointment with RIKEN. He has been studying the working mechanisms and the physiological functions of a molecular motor, kinesin by the combination of wide variety of imaging techniques such as single molecule imaging, cryo-EM, X-ray crystallography, and fluorescent live cell imaging.|